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Antimicrobial Susceptibility Pattern of Enterococcus Species Isolated From Clinical Samples in South India

http://advancejournals.org/journal-of-recent-advances-in-applied-sciences/wp-content/uploads/sites/30/2016/06/2726-10.pdf

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 Enterococci are common cause of hospital acquired infection and have become progressively more resistant to antibiotic. Among the 13 Enterococcus species ninety percentage of Enterococcal infection are due to Enterococcus faecalis .E. faecalis is a causative agent for nosocomial infection. The present study was carried out to asses the changing trends in antibiotic susceptibility of Enterococcus species isolated from clinical samples from Gulbarga region.122 samples were collected from various hospitals and diagnostic centers in Gulbarga region. The clinical isolates were identified by Facklam and Collins conventional method. The susceptibility tests were done against vancomycin, ampicillin, oxacillin, rifamycin, ciprofloxacin, tobramycin, gentamycin, teicoplanin and streptomycin by Kirby bauer method. MIC of resistant E. faecalis isolates were determined by NCCLS method. In our study, E. faecalis strains found to be 50% multi-drug resistant and the resistance was more in case of vancomycin antibiotic. The E. faecium isolates showed high resistance to gentamycin and streptomycin among the few MDR strains. E. durans were susceptible to all the antibiotic expect streptomycin and E. gallinarium were found susceptible to all antibiotics. The MIC’s determined of the 12 strains, showed the prevalence of HLAR and HLGR among the E .faecalis isolates. The present study reveals for the first time emergence of vanocmycin resistant enterococci from this part of world and indicates the magnitude of antibiotic resistance in and around the study area.

Healing of Bone Defects by Autogenous Platelet Rich Plasma in Pediatric Patients

http://advancejournals.org/journal-of-recent-advances-in-applied-sciences/wp-content/uploads/sites/30/2016/06/27311-13.pdf

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 OBJECTIVES – Objective of this study is to evaluate the new bone formation in bony defects after placement of PRP and its efficacy of regenerative potential .

METHOD – Twenty five pediatric patients were selected for the study and PRP was extracted from patients own blood and placed in defect in all patients. Post operative clinical and radiographic observation was done at 1st,4th and 6 th month.

RESULTS – Faster bone healing in all patients was observed.

CONCLUSION – It can be concluded by the study that PRP is a better source of bone induction growth factors and regeneration of bone can be done at faster rate which is a favorable biological response similar to natural healing process

Management of a Young Edentulous Patient Having Papillon Lefévre Syndrome – a Case Report

http://advancejournals.org/journal-of-recent-advances-in-applied-sciences/wp-content/uploads/sites/30/2016/06/27519-23.pdf

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 Papillon-Lefevre syndrome is a rare autosomal recessive disorder in which the frequently observed manifestations are palmoplantar keratinization and premature loss of both deciduous and permanent teeth. The palmoplantar keratoderma typically has its onset between the ages of 1 and 4 years and severe periodontitis starts at the age of 3 or 4 years. The deciduous teeth frequently fall out by about age five and most of the permanent teeth may also be lost by approximately age 17. In the present, a young, edentulous male of this syndrome having most the characteristic features was treated by modified Complete Denture prosthesis considering his young age and low socioeconomic status. 

Characterization of Physical Factors for Optimum Lovastatin Production by Aspergillus Terreus Klvb28mu21 Under Solid State Fermentation

http://advancejournals.org/journal-of-recent-advances-in-applied-sciences/wp-content/uploads/sites/30/2016/06/27101-05.pdf

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 Lovastatin a hypocholesterolemic agent, competitively inhibits the rate limiting enzyme of cholesterol biosynthesis 3-hydroxy-3-glutaryl glutaryl coenzyme A (HMG-CoA) reductase, which catalyzes the reduction of HMG-CoA to mevalonate during cholesterol biosynthesis. We studied lovastatin production by Aspergillus terreus KLVB28mu21 strain employing Solid State Fermentation of wheat bran. Highest yield (1110 g/gm of DW of substrate) of lovastatin was obtained at moisture content 65%, pH 5.5, temperature 300C, inoculum size 1×108 spores mL-1 and bed depth 2cm.