Medicinal properties of many Indian herbal plants are yet to be evaluated. The aim of this study was to determine the phytochemical constituents, antibacterial activity, cytopathic and cytotoxicity effects of Tylophora indica, Curcuma amada and Urtica dioica. Various parts of the plants were collected and shade dried. The solvents used for extraction of plants were water, ethanol, methanol, petroleum ether and chloroform. In vitro antibacterial activity was performed by agar disc diffusion method. Normal and multi drug resistant bacterial strains of E.coli, P.aeruginosa were used. Curcuma amada was found to exhibit highest toxicity against both the bacterial strains. But none of the plant exhibited activity over multi drug resistant bacterial strains. Further in vitro cytopathic and cytotoxic effects were determined for Tylophora indica, Curcuma amada and Urtica dioica upon BHK-21 fibroblast cell lines. Cytotoxicity of the plant extracts were determined by calculating the IC50 values. The highest cytotoxicity was found in alcoholic extracts obtained from Tylophora indica with IC50 value ~20µg/ml respectively.